polyclonal goat anti-mouse cxcl16 ab  (PeproTech)

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT.

doi: 10.4049/jimmunol.176.1.43

Figure Lengend Snippet: FIGURE 1. Chemokine mRNA expression profiles were analyzed with the mouse chemokines and receptors gene array and compared between the FAE (A) and the IECs (B). Among the 32 chemokines tested, 13 chemo- kines were detected in either FAE or IECs. FAE chemokine expression was characterized by the increase in CCL9/MIP- (1), CCL20/MIP-3 (2), and CXCL16 (3) and by the decrease in CCL5/RANTES (4), CCL25/thymus- expressed chemokine (5), and CX3CL1/fractralkine (6). The precise gene list is available upon request or directly from the web site www.superarray.com.

Article Snippet: The sections were incubated with 0.5% blocking buffer (Roche) in PBS for 30 min at room temperature and then with 2 g/ml goat anti-mouse CXCL16 polyclonal Ab (R&D Systems) or an identical concentration of control goat IgG overnight at 4°C.

Techniques: Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT.

doi: 10.4049/jimmunol.176.1.43

Figure Lengend Snippet: FIGURE 2. Quantitative real-time PCR analysis of chemokine mRNA expression on the FAE and IECs (Sm.Int., small intestine). The FAE and IECs from the small intestine and the colon of specific pathogen-free mice are described in Materials and Methods. The mRNA expression levels of CXCL16, CCL9, and CCL20 were normalized to that of GAPDH and expressed as the fold change relative to the normalized value in the colonic epithelium. Data are mean SE of three different experiments.

Article Snippet: The sections were incubated with 0.5% blocking buffer (Roche) in PBS for 30 min at room temperature and then with 2 g/ml goat anti-mouse CXCL16 polyclonal Ab (R&D Systems) or an identical concentration of control goat IgG overnight at 4°C.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT.

doi: 10.4049/jimmunol.176.1.43

Figure Lengend Snippet: FIGURE 3. CXCL16 mRNA expression on the FAE. In situ hybridiza- tion was performed with CXCL16-specific antisense (A and B) or control sense (C and D) probe. A positive signal for CXCL16 mRNA was observed in the FAE (arrowheads) of murine PPs but not in the villous epithelium (arrows). Scale bars, 200 (A and C) or 50 (B and D) m.

Article Snippet: The sections were incubated with 0.5% blocking buffer (Roche) in PBS for 30 min at room temperature and then with 2 g/ml goat anti-mouse CXCL16 polyclonal Ab (R&D Systems) or an identical concentration of control goat IgG overnight at 4°C.

Techniques: Expressing, In Situ, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT.

doi: 10.4049/jimmunol.176.1.43

Figure Lengend Snippet: FIGURE 4. CXCL16 immuno- staining of murine small intestine. A–F, Sections were stained with anti- CXCL16 polyclonal Ab (A–C) or control goat IgG (D–F). Positive staining was observed throughout the FAE (A and B) but not in the villous epithelium (C). G–I, confocal images of CXCL16 (green) and UEA-1 (red) staining, with DAPI (white) as counter nuclei staining. The merged image (I) clearly shows CXCL16 ex- pression on the basolateral plasma membrane of M cells (arrowheads) and the other FAE cells. Scale bars, 100 (A, C, D, and F), 50 (E), or 20 (G–I) m.

Article Snippet: The sections were incubated with 0.5% blocking buffer (Roche) in PBS for 30 min at room temperature and then with 2 g/ml goat anti-mouse CXCL16 polyclonal Ab (R&D Systems) or an identical concentration of control goat IgG overnight at 4°C.

Techniques: Immunostaining, Staining, Control, Clinical Proteomics, Membrane

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT.

doi: 10.4049/jimmunol.176.1.43

Figure Lengend Snippet: FIGURE 5. CXCL16 immunostaining of various inductive sites of the GALTs. Tissue sections containing duodenal ILFs (A and B) or cecal patches (C and D) of SPF mouse or human ileal lymphoid follicles (E–H) were immunostained with goat anti-murine (A and C), anti-human (E and G) CXCL16 antibodies, or control IgG (B, D, F, and H). Scale bars rep- resent 100 mm.

Article Snippet: The sections were incubated with 0.5% blocking buffer (Roche) in PBS for 30 min at room temperature and then with 2 g/ml goat anti-mouse CXCL16 polyclonal Ab (R&D Systems) or an identical concentration of control goat IgG overnight at 4°C.

Techniques: Immunostaining, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT.

doi: 10.4049/jimmunol.176.1.43

Figure Lengend Snippet: FIGURE 6. CXCL16 expression by FAE under GF and CV conditions. A, Total RNA was prepared from the PP FAE in mice maintained at GF or CV condition and analyzed for the CXCL16 expression by real-time PCR. The levels were normalized to those of GAPDH. Data are described as the fold change relative to the normalized value in small intestinal epithelium at CV condition. Mean value SD of four samples obtained from different mice are shown. Sm.Int., small intestine. B, CXCL16 protein expression in GF mice was confirmed by immunohistochemistry. Tissue sections con- taining PPs of GF mice were immunostained with anti-CXCL16 Ab (upper panel) or control IgG (lower panel). Scale bars represent 100 mm.

Article Snippet: The sections were incubated with 0.5% blocking buffer (Roche) in PBS for 30 min at room temperature and then with 2 g/ml goat anti-mouse CXCL16 polyclonal Ab (R&D Systems) or an identical concentration of control goat IgG overnight at 4°C.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunohistochemistry, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT.

doi: 10.4049/jimmunol.176.1.43

Figure Lengend Snippet: FIGURE 7. Analysis of CXCR6/Bonzo-expressing cells in PP lymphocytes. A, Various cell populations in murine PPs were stained with CXCL16-Fc fusion protein (u) or control IgG (). Activated T cells were prepared by in vitro stimulation with anti-CD3 and CD28 mAbs. B, CD4 T cells were divided into CXCR6 and CXCR6 populations and further analyzed for CD44 and CD62L expression.

Article Snippet: The sections were incubated with 0.5% blocking buffer (Roche) in PBS for 30 min at room temperature and then with 2 g/ml goat anti-mouse CXCL16 polyclonal Ab (R&D Systems) or an identical concentration of control goat IgG overnight at 4°C.

Techniques: Expressing, Staining, Control, In Vitro

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT.

doi: 10.4049/jimmunol.176.1.43

Figure Lengend Snippet: FIGURE 8. CXCL16 mediates migration and adhesion of activated PP T cells. A, Dose-dependent effect of CXCL16 on migration of in vitro activated PP CD4 (E) and CD8 (F) T cells. B, chemotactic activity of activated PP CD4 and CD8 T cells were performed with various che- mokines that are expressed in the distinct regions of PPs at the concentra- tion of 10 () or 100 (u) ng/ml. C, Adhesion of activated PP T cells to mCXCL16-expressing (u) or wild-type () CHO cells. Chemotaxis and cell adhesion assays were repeated at least three times, and basically sim- ilar results were obtained each time.

Article Snippet: The sections were incubated with 0.5% blocking buffer (Roche) in PBS for 30 min at room temperature and then with 2 g/ml goat anti-mouse CXCL16 polyclonal Ab (R&D Systems) or an identical concentration of control goat IgG overnight at 4°C.

Techniques: Migration, In Vitro, Activity Assay, Expressing, Chemotaxis Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT.

doi: 10.4049/jimmunol.176.1.43

Figure Lengend Snippet: FIGURE 10. Effect of anti-CXCL16 neutralizing mAb on T cell localization in PPs. and , Anti-CXCL16 neutralizing mAb (B) or control IgG (A) was injected into mice before and after transferring in vitro activated T cells that are labeled with PKH red dye. Tissue sections of PPs at 16 h post-transfer. Sections were counterstained with DAPI (blue). Scale bar represents 100 m. C and D, T cells in the SED and the IFR were counted, and the number was normalized to field. Values are mean SD of four different samples obtained from different mice.

Article Snippet: The sections were incubated with 0.5% blocking buffer (Roche) in PBS for 30 min at room temperature and then with 2 g/ml goat anti-mouse CXCL16 polyclonal Ab (R&D Systems) or an identical concentration of control goat IgG overnight at 4°C.

Techniques: Control, Injection, Transferring, In Vitro, Labeling